Plasmid

Part:BBa_K4161601

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-09-27)


pNZ8148

pNZ8148 is a 3167bp plasmid that can be transformed into L.lactis and L.bacillus. The selection marker is Chloramphenicol-resistance. The promoter is Usp45 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 235
    Illegal SpeI site found at 229
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 229
    Illegal PstI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 235
    Illegal SpeI site found at 229
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 235
    Illegal SpeI site found at 229
    Illegal PstI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None